Exploiting mammalian low-complexity domains for liquid-liquid phase separation–driven underwater adhesive coatings

Many biological materials form via liquid-liquid phase separation (LLPS), followed by maturation into a solid-like state. Here, using a biologically inspired assembly mechanism designed to recapitulate these sequential assemblies, we develop ultrastrong underwater adhesives made from engineered proteins containing mammalian low-complexity (LC) domains. We show that LC domain–mediated LLPS and maturation substantially promotes the wetting, adsorption, priming, and formation of dense, uniform amyloid nanofiber coatings on diverse surfaces (e.g., Teflon), and even penetrating difficult-to-access locations such as the interiors of microfluidic devices. Notably, these coatings can be deposited on substrates over a broad range of pH values (3 to 11) and salt concentrations (up to 1 M NaCl) and exhibit strong underwater adhesion performance. Beyond demonstrating the utility of mammalian LC domains for driving LLPS in soft materials applications, our study illustrates a powerful example of how combining LLPS with subsequent maturation steps can be harnessed for engineering protein-based materials. Underwater adhesive systems in many marine organisms are known to form coacervates via liq...

The Inlabtec TA12 Serial Diluter was developed in response to the demand from food culture and

The Inlabtec TA12 Serial Diluter was developed in response to the demand from food culture and probiotic producers undertaking final quality control with the standard plate count method. Because you are placing the sample into a sterile, empty Serial Dilution Bag instead of a prefilled test tube, then adding the diluent at the touch of a button, performing serial dilutions with the TA12 is fast, reliable, and much less taxing than traditional procedures. The TA12 combines two lines of six dilution bags in parallel, and the telescopic dispensing arm makes changing between the two rows of bags easy and convenient. Any kind of diluent can be used, enabling probiotic producers to use preferred diluents such as skimmed milk, for which prefilled test tubes are not available. Serial Dilution Bags are made from high-purity polyethylene, guaranteed to be free of any substances that could inhibit proliferation of sensitive cells. AMS Biotechnology offers an extensive portfolio of Poly (ADP-ribose) polymerase (PARP) isozymes such as assay kits, inhibitors, recombinant histone substrates, and screening/profiling services for over 10 different PARPs. PARPs play key signaling roles in apoptos...